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melanocyte growth basal medium 4 (mbm-4)  (Lonza)


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    Lonza melanocyte growth basal medium 4 (mbm-4)
    Melanocyte Growth Basal Medium 4 (Mbm 4), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/melanocyte+basal+medium/melanocyte+cell+basal+medium+4/pmc11865835-71-0-6
    Average 90 stars, based on 1 article reviews
    melanocyte growth basal medium 4 (mbm-4) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: A chemical compound from fruit extract of Juglans mandshurica inhibits melanogenesis through p-ERK-associated MITF degradation.
    Article Snippet: Background: Trials for regulation of abnormal hyperpigmentation from the use of natural products have been going on for years.. Leaf and root extracts from Juglans mandshurica are reported to function as antioxidants and to suppress allergic dermatitis.. However, studies evaluating its fruit extract and the chemical compounds from the fruit extract are lacking in dermatology fields, including melanogenesis.

    other:

    Article Title: Impact of ultraviolet radiation on dermal and epidermal DNA damage in a human pigmented bilayered skin substitute.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1002/term.2959 This article is protected by copyright.. All rights reserved.

    Cell Culture:

    Article Title: 7-desacetoxy-6,7-dehydrogedunin discovered by high-throughput screening system suppresses melanogenesis through ATP-P2X7 signaling inhibition.
    Article Snippet: Background: Hyperpigmented skin disorders such as melasma and lentigo are common photoaging diseases that cause cosmetic problems.. The pigmentation is usually exacerbated by ultraviolet (UV) radiation, and various factors and pathways are involved in UV-mediated melanogenesis.. Adenosine 5′-triphosphate (ATP), a well-known molecular unit of intracellular energy, is also regarded as a mediator of UV-mediated melanogenesis via the P2X7 purinergic receptor.



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    Sequential Irradiation and Medium Exchange in <t>Melanocyte</t> Culture ± Keratinocyte-Conditioned Medium for Pro-Melanogenic Enzyme Analysis. Primary keratinocytes (Population 1) were cultured separately on a feeder layer of gamma-irradiated human fibroblasts and irradiated on days 3, 5, and 7 using an LED module with specified light parameters (10 mW/cm 2 , 15 J/cm 2 ). The culture medium was collected prior to irradiation to allow α-MSH accumulation over several hours and because cells were irradiated in PBS to prevent photon absorption and ROS generation. The collected medium was stored at −80°C and later concentrated 10-fold. Melanocytes (Populations 1, 3, and 4), seeded in keratinocyte medium with FGF2, received thawed keratinocyte-conditioned medium at 1X concentration post each irradiation. Three types of conditioned medium were used: CM (keratinocyte medium only), CMK (medium from non-irradiated keratinocytes), and CMiK (medium from irradiated keratinocytes). On day 12, melanocytes were harvested and analyzed via Western blot for TYR and DCT, marking the analysis of pro-melanogenic activity across different light and media conditions. I = Irradiated, NI = non-irradiated, Sham = non-irradiated cell culture, KCM = keratinocyte-conditioned medium, WB = Western blot. This figure was sketched using BioRender.
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    Image Search Results


    Sequential Irradiation and Medium Exchange in Melanocyte Culture ± Keratinocyte-Conditioned Medium for Pro-Melanogenic Enzyme Analysis. Primary keratinocytes (Population 1) were cultured separately on a feeder layer of gamma-irradiated human fibroblasts and irradiated on days 3, 5, and 7 using an LED module with specified light parameters (10 mW/cm 2 , 15 J/cm 2 ). The culture medium was collected prior to irradiation to allow α-MSH accumulation over several hours and because cells were irradiated in PBS to prevent photon absorption and ROS generation. The collected medium was stored at −80°C and later concentrated 10-fold. Melanocytes (Populations 1, 3, and 4), seeded in keratinocyte medium with FGF2, received thawed keratinocyte-conditioned medium at 1X concentration post each irradiation. Three types of conditioned medium were used: CM (keratinocyte medium only), CMK (medium from non-irradiated keratinocytes), and CMiK (medium from irradiated keratinocytes). On day 12, melanocytes were harvested and analyzed via Western blot for TYR and DCT, marking the analysis of pro-melanogenic activity across different light and media conditions. I = Irradiated, NI = non-irradiated, Sham = non-irradiated cell culture, KCM = keratinocyte-conditioned medium, WB = Western blot. This figure was sketched using BioRender.

    Journal: Frontiers in Physiology

    Article Title: Balancing act: optimizing blue light for melanogenesis while minimizing cellular damage in primary human skin cells

    doi: 10.3389/fphys.2024.1513054

    Figure Lengend Snippet: Sequential Irradiation and Medium Exchange in Melanocyte Culture ± Keratinocyte-Conditioned Medium for Pro-Melanogenic Enzyme Analysis. Primary keratinocytes (Population 1) were cultured separately on a feeder layer of gamma-irradiated human fibroblasts and irradiated on days 3, 5, and 7 using an LED module with specified light parameters (10 mW/cm 2 , 15 J/cm 2 ). The culture medium was collected prior to irradiation to allow α-MSH accumulation over several hours and because cells were irradiated in PBS to prevent photon absorption and ROS generation. The collected medium was stored at −80°C and later concentrated 10-fold. Melanocytes (Populations 1, 3, and 4), seeded in keratinocyte medium with FGF2, received thawed keratinocyte-conditioned medium at 1X concentration post each irradiation. Three types of conditioned medium were used: CM (keratinocyte medium only), CMK (medium from non-irradiated keratinocytes), and CMiK (medium from irradiated keratinocytes). On day 12, melanocytes were harvested and analyzed via Western blot for TYR and DCT, marking the analysis of pro-melanogenic activity across different light and media conditions. I = Irradiated, NI = non-irradiated, Sham = non-irradiated cell culture, KCM = keratinocyte-conditioned medium, WB = Western blot. This figure was sketched using BioRender.

    Article Snippet: Melanocytes were isolated from keratinocytes by culturing the epidermal cells for 2 days in melanocyte medium (Melanocyte Basal Medium [Lonza, Basel, Switzerland]) supplemented with 0.5% fetal bovine serum (HyClone, Wilmington, DE, United States), calcium, bovine pituitary extract, recombinant human basic fibroblast growth factor, recombinant human insulin, hydrocortisone, phorbol myristate acetate, penicillin, and gentamicin, along with 0.1 mg/mL geneticin (G418; Sigma Chemicals, St. Louis, MO, United States).

    Techniques: Irradiation, Cell Culture, Concentration Assay, Western Blot, Activity Assay

    Keratinocyte cultures were exposed to various light parameters across three time points: day 3 (white bars), day 5 (gray bars), and day 7 (black bars). (A) Effects of Different Irradiation Parameters on Primary Keratinocyte Viability. This bar graph presents the results of the Alamar Blue (AB) assay conducted on primary keratinocyte monolayers. The x -axis displays different combinations of irradiance (mW/cm 2 ) and fluence (J/cm 2 ) for blue light, with the last combination representing UVA light at 15 mW/cm 2 and 20 J/cm 2 . The y -axis indicates the fold change in cell viability relative to sham control (untreated cells). (B) Alpha-MSH Levels in Keratinocyte Cultures Exposed to Various Light Parameters. This graph shows the fold change in alpha-Melanocyte Stimulating Hormone (α-MSH) levels, measured by ELISA. The x -axis lists the combinations of irradiance (mW/cm 2 ) and fluence (J/cm 2 ) for blue light, with the last combination representing UVA light at 15 mW/cm 2 and 20 J/cm 2 . The y -axis displays the fold change in α-MSH levels compared to the sham control (untreated cells). Error bars represent the standard deviation (SD) among the three distinct cell populations (N = 3) analyzed. Statistically significant differences between days for each treatment condition are marked by crosses ( † for p < 0.05, †† for p < 0.01). Statistical differences between a condition (i.e., day and light treatment) and its respective sham are marked by asterisks (* for p < 0.05, ** for p < 0.01, *** for p < 0.001).

    Journal: Frontiers in Physiology

    Article Title: Balancing act: optimizing blue light for melanogenesis while minimizing cellular damage in primary human skin cells

    doi: 10.3389/fphys.2024.1513054

    Figure Lengend Snippet: Keratinocyte cultures were exposed to various light parameters across three time points: day 3 (white bars), day 5 (gray bars), and day 7 (black bars). (A) Effects of Different Irradiation Parameters on Primary Keratinocyte Viability. This bar graph presents the results of the Alamar Blue (AB) assay conducted on primary keratinocyte monolayers. The x -axis displays different combinations of irradiance (mW/cm 2 ) and fluence (J/cm 2 ) for blue light, with the last combination representing UVA light at 15 mW/cm 2 and 20 J/cm 2 . The y -axis indicates the fold change in cell viability relative to sham control (untreated cells). (B) Alpha-MSH Levels in Keratinocyte Cultures Exposed to Various Light Parameters. This graph shows the fold change in alpha-Melanocyte Stimulating Hormone (α-MSH) levels, measured by ELISA. The x -axis lists the combinations of irradiance (mW/cm 2 ) and fluence (J/cm 2 ) for blue light, with the last combination representing UVA light at 15 mW/cm 2 and 20 J/cm 2 . The y -axis displays the fold change in α-MSH levels compared to the sham control (untreated cells). Error bars represent the standard deviation (SD) among the three distinct cell populations (N = 3) analyzed. Statistically significant differences between days for each treatment condition are marked by crosses ( † for p < 0.05, †† for p < 0.01). Statistical differences between a condition (i.e., day and light treatment) and its respective sham are marked by asterisks (* for p < 0.05, ** for p < 0.01, *** for p < 0.001).

    Article Snippet: Melanocytes were isolated from keratinocytes by culturing the epidermal cells for 2 days in melanocyte medium (Melanocyte Basal Medium [Lonza, Basel, Switzerland]) supplemented with 0.5% fetal bovine serum (HyClone, Wilmington, DE, United States), calcium, bovine pituitary extract, recombinant human basic fibroblast growth factor, recombinant human insulin, hydrocortisone, phorbol myristate acetate, penicillin, and gentamicin, along with 0.1 mg/mL geneticin (G418; Sigma Chemicals, St. Louis, MO, United States).

    Techniques: Irradiation, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Relative protein expression of Tyrosinase (Tyr) and Dopachrome Tautomerase (DCT) normalized to β-actin in non-irradiated and irradiated primary melanocytes cultured with or without keratinocyte-conditioned medium. (A) Tyrosinase Protein Expression: Bar graphs show fold change in Tyr levels in non-irradiated (left) and irradiated (right) melanocytes. Conditions include melanocytes without keratinocyte-conditioned medium (CM), with conditioned medium from non-irradiated keratinocytes (CM-K), and irradiated keratinocytes (CM-iK). Significant increases in Tyr expression were observed in non-irradiated melanocytes exposed to irradiated keratinocyte-conditioned medium (CM-iK) ( p < 0.05). Immunoblot images of Tyr and β-actin are shown below the bar graphs. (B) Dopachrome Tautomerase (DCT) Protein Expression: Bar graphs present the fold change in DCT levels in non-irradiated (left) and irradiated (right) melanocytes. A significant increase in DCT expression is seen in non-irradiated melanocytes exposed to irradiated keratinocyte-conditioned medium ( p < 0.05). Immunoblot images of DCT and β-actin are shown below the bar graphs. Error bars indicate the standard deviation (SD) between the three populations of melanocytes (N = 3) assessed, and p < 0.05 is considered statistically significant.

    Journal: Frontiers in Physiology

    Article Title: Balancing act: optimizing blue light for melanogenesis while minimizing cellular damage in primary human skin cells

    doi: 10.3389/fphys.2024.1513054

    Figure Lengend Snippet: Relative protein expression of Tyrosinase (Tyr) and Dopachrome Tautomerase (DCT) normalized to β-actin in non-irradiated and irradiated primary melanocytes cultured with or without keratinocyte-conditioned medium. (A) Tyrosinase Protein Expression: Bar graphs show fold change in Tyr levels in non-irradiated (left) and irradiated (right) melanocytes. Conditions include melanocytes without keratinocyte-conditioned medium (CM), with conditioned medium from non-irradiated keratinocytes (CM-K), and irradiated keratinocytes (CM-iK). Significant increases in Tyr expression were observed in non-irradiated melanocytes exposed to irradiated keratinocyte-conditioned medium (CM-iK) ( p < 0.05). Immunoblot images of Tyr and β-actin are shown below the bar graphs. (B) Dopachrome Tautomerase (DCT) Protein Expression: Bar graphs present the fold change in DCT levels in non-irradiated (left) and irradiated (right) melanocytes. A significant increase in DCT expression is seen in non-irradiated melanocytes exposed to irradiated keratinocyte-conditioned medium ( p < 0.05). Immunoblot images of DCT and β-actin are shown below the bar graphs. Error bars indicate the standard deviation (SD) between the three populations of melanocytes (N = 3) assessed, and p < 0.05 is considered statistically significant.

    Article Snippet: Melanocytes were isolated from keratinocytes by culturing the epidermal cells for 2 days in melanocyte medium (Melanocyte Basal Medium [Lonza, Basel, Switzerland]) supplemented with 0.5% fetal bovine serum (HyClone, Wilmington, DE, United States), calcium, bovine pituitary extract, recombinant human basic fibroblast growth factor, recombinant human insulin, hydrocortisone, phorbol myristate acetate, penicillin, and gentamicin, along with 0.1 mg/mL geneticin (G418; Sigma Chemicals, St. Louis, MO, United States).

    Techniques: Expressing, Irradiation, Cell Culture, Western Blot, Standard Deviation

    Microscopic images of melanocytes (population 1) cultured in conditioned media from keratinocytes (CM, CMK, CMiK) before lysis for Western blot analysis on day 12. Representative images of melanocytes cultured in CM (keratinocyte medium only), CMK (conditioned medium from non-irradiated keratinocytes), and CMiK (conditioned medium from irradiated keratinocytes) are shown for non-irradiated (left panels) and irradiated (right panels) melanocytes. Images were captured at ×10 magnification. Melanocytes in CM show a healthy morphology with well-defined spindle shapes and minimal pigmentation. Those cultured in CMK exhibit slightly more spindle elongation with no noticeable pigmentation changes. Melanocytes cultured in CMiK display enhanced pigmentation, particularly in irradiated conditions, consistent with increased pro-melanogenic factor activity. These photomicrographs were obtained from live-cell cultures without fixation, eliminating the possibility of formalin or other pigment artifacts. Scale bars = 400 µm.

    Journal: Frontiers in Physiology

    Article Title: Balancing act: optimizing blue light for melanogenesis while minimizing cellular damage in primary human skin cells

    doi: 10.3389/fphys.2024.1513054

    Figure Lengend Snippet: Microscopic images of melanocytes (population 1) cultured in conditioned media from keratinocytes (CM, CMK, CMiK) before lysis for Western blot analysis on day 12. Representative images of melanocytes cultured in CM (keratinocyte medium only), CMK (conditioned medium from non-irradiated keratinocytes), and CMiK (conditioned medium from irradiated keratinocytes) are shown for non-irradiated (left panels) and irradiated (right panels) melanocytes. Images were captured at ×10 magnification. Melanocytes in CM show a healthy morphology with well-defined spindle shapes and minimal pigmentation. Those cultured in CMK exhibit slightly more spindle elongation with no noticeable pigmentation changes. Melanocytes cultured in CMiK display enhanced pigmentation, particularly in irradiated conditions, consistent with increased pro-melanogenic factor activity. These photomicrographs were obtained from live-cell cultures without fixation, eliminating the possibility of formalin or other pigment artifacts. Scale bars = 400 µm.

    Article Snippet: Melanocytes were isolated from keratinocytes by culturing the epidermal cells for 2 days in melanocyte medium (Melanocyte Basal Medium [Lonza, Basel, Switzerland]) supplemented with 0.5% fetal bovine serum (HyClone, Wilmington, DE, United States), calcium, bovine pituitary extract, recombinant human basic fibroblast growth factor, recombinant human insulin, hydrocortisone, phorbol myristate acetate, penicillin, and gentamicin, along with 0.1 mg/mL geneticin (G418; Sigma Chemicals, St. Louis, MO, United States).

    Techniques: Cell Culture, Lysis, Western Blot, Irradiation, Activity Assay